Pluripotent teratocarcinoma--Simian virus 40 transformed mouse fibroblasts somatic cell hybrids.

Tumorigenicity of simian virus 40-transformed human cells and mouse--human hybrids in nude mice.

Four different human cell lines transformed by simian virus 40 (SV40) were tested for their tumorigenicity in athymic nude mice. Two of these lines, W18Va2 and GM52VA, were found to be tumorigenic when inoculated at a concentration of 1 x 10(7) cells per mouse. The other two cell lines, LN-SV and GM54VA, were found to induce very small tumors only after the injection of approximately 1 x 10(8) ...

Suppression of the simian virus 40 tumorigenic phenotype in hybrid cells formed from simian virus 40- and adenovirus 2-transformed hamster embryo cells.

Hamster cells transformed by adenovirus 2 (Ad2) or simian virus 40 (SV40) have different tumorigenic phenotypes. In the present study, somatic cell hybrids formed from Ad2- and SV40-transformed hamster cells were used to determine whether possible interactions between the integrated viral genomes would influence the tumorigenic phenotype of hybrid transformed cells. These somatic cell hybrids w...

Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.

We have produced somatic cell hybrids between mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase IMP: pyrophosphate phosphoribosyltransferase; EC 2.4.2.8) and spleen cells derived from mice primed with either syngeneic or allogeneic cells transformed by simian virus 40. Such hybrids produced antibodies specific for simian virus 40 tumor (T) antigen. Only four of twelve inde...

Somatic Cell Hybrids between Mouse Peritoneal Macrophages and Sv40-transformed Human Cells

Fusion of mouse peritoneal macrophages with SV40-transformed human cells, deficient in hypoxanthine guanine phosphoribosyltransferase, resulted in the formation of transformed somatic cell hybrids which contained, without exception, the human chromosome 7 carrying the SV40 genome. It is postulated that the hybridization of mouse nondividing cells with human cancer cells could permit the identif...

Ultrastructural Localization of Concanavalin a Receptors in Somatic Cell Hybrids between Normal Mouse Cells and Simian Virus 40-transformed Rat Cells